--- title: "Adding external data to ggseg plotting" output: rmarkdown::html_vignette vignette: > %\VignetteIndexEntry{Adding external data to ggseg plotting} %\VignetteEngine{knitr::rmarkdown} \usepackage[utf8]{inputenc} --- ```{r setup, include=F} knitr::opts_chunk$set(eval=TRUE, fig.retina = 3) ``` ## Introduction Once you have covered the main functionality in `ggseg` you will want to use it to plot the results of your data. In order to do this, your data must adhere to certain specifications, so that `ggseg` can manage to merge your data with the atlas you are using. This means you need to be able to inspect and locate the way the regions you are working with are names in the internal atlas files. This vignette should provide the tools you need to figure these features out, and to manipulate your data to fit these requirements. ## Inspecting the atlas labels There are several ways you can inspect what the data in the atlas looks like. While each atlas has some small differences, they all share six main columns: **1. .long** - x-axis **2. .lat **- y-axis **3. .region **- name of region/network **4. .hemi** - hemisphere (left or right) **5. .side** - side of view (medial, lateral, sagittal or axial) Most atlases also have a `label` column, which are raw names assigned from the program run to segment/extract data. TO inspect the atlases, call them in the console. ```{r start} library(ggseg) library(dplyr) library(ggplot2) dk ``` Further inspection of the atlas data can be explored by turning them into tibbles (or data.frames) ```{r} as_tibble(dk) ``` Here you can see information about the `dk` atlas, and the main attributes of this atlas. If you want to use external data with your `ggseg` plot, you will need to make sure that your data has at least one column corresponding in name and content with another in the atlas you are using. ## Structuring data for merging For instance, here we make some data for the "default" and "visual" networks in the `dk` atlas, and two p values for those two networks. ```{r} someData = tibble( region=c("superior temporal","precentral", "lateral orbitofrontal"), p=c(.03,.6, .05) ) someData ``` Notice you we have spelled both the column name and the region names **exactly** as they appear in the data. This is necessary for the merging within the `ggseg` function to work properly. This merge can be attempted before supplying the data to `ggseg` to see if there are any errors. ```{r} dk %>% as_tibble() %>% left_join(someData) ``` No errors! Yes, the `p` column is seemingly full of `NA`s, but that is just because the top of the data is the somatomotor network, which we did not supply any p values for, so it has been populated with `NA`s. We can sort the data differently, so we can see the `p`has been added correctly. ```{r} dk %>% as_tibble() %>% left_join(someData) %>% arrange(p) ``` If you need your data to be matched on several columns, the approach is the same. Add the column you want to match on, with the **exact** same name, and make sure it's content matches the content of the same column in the data. ```{r} someData$hemi = rep("left", nrow(someData)) someData dk %>% as_tibble() %>% left_join(someData) %>% arrange(p) ``` Notice how the message now states that it is joining `by = c("region", "hemi")`. The merge function has recognized that there are two equally named columns, and assumes (in this case correctly) that these are equivalent. **Notice** that everything is case-sensitive, so writing `Region` or `Left` will not result in matching. ## Providing data to `ggseg` When you have managed to create data that merges nicely with the atlas, you can go ahead and supply it to the function. ```{r} ggplot(someData) +geom_brain( atlas=dk, mapping=aes(fill=p)) ``` You can actually also supply it directly as an atlas. For instance, if you had saved the merged data from the previous steps, you can supply this directly to the `atlas` option. ```{r} newAtlas = dk %>% as_tibble() %>% left_join(someData) %>% as_brain_atlas() ggplot() + geom_brain(atlas = newAtlas, mapping = aes(fill=p), position = position_brain(hemi ~ side) ) ``` It is this possibility of supplying a custom atlas that gives you particular flexibility, though a little tricky to begin with. Lets do a recap of the unwanted results: ```{r datasupp3} someData = data.frame( region = rep(c("transverse temporal", "insula", "precentral","superior parietal"),2), p = sample(seq(0,.5,.001), 8), AgeG = c(rep("Young",4), rep("Old",4)), stringsAsFactors = FALSE) ggplot(someData) + geom_brain(atlas = dk, colour="white", mapping=aes(fill=p)) + facet_wrap(~AgeG, ncol=1) + theme(legend.position = "bottom") ``` See how you have three facets, when you only have 2 groups, and that the "background" brain is not printed in your two groups. This is because for ggplot, that is what the data looks like. For this to work, you can supply already grouped data to `ggseg`, but you must make sure they are grouped by the columns you will use for faceting, or else it will not work. ```{r duplicate} # If you group_by the columns you will facet by, this will work well. someData = someData %>% group_by(AgeG) # We can now supply the newAtlas as an atlas to ggseg ggplot(someData) + geom_brain(atlas=dk, colour="white", mapping=aes(fill=p)) + facet_wrap(~AgeG, ncol=1) + theme(legend.position = "bottom") + scale_fill_gradientn(colours = c("royalblue","firebrick","goldenrod"),na.value="grey") ``` This whole procedure can be piped together, so you don't have to save all the intermediate steps. ```{r duplicatepipe} someData %>% group_by(AgeG) %>% ggplot() + geom_brain(atlas=dk, colour="white", mapping=aes(fill=p)) + facet_wrap(~AgeG, ncol=1) + theme(legend.position = "bottom") + scale_fill_gradientn(colours = c("royalblue","firebrick","goldenrod"),na.value="grey") ```